HIM GEM-X Experiment Calculator v2.0

Compare workflows, estimate costs, and plan your single-cell experiments | Generated January 16, 2026

Shared Settings

HIM Service Pricing

Cancer Center Discount: UChicago Comprehensive Cancer Center members receive 20% off all HIM service fees. Contact HIM for pricing details.
Library Calculation Rules:
• Singleplex/Multiome: 8 samples/chip, 1 library per sample (1:1 ratio)
• OCM: 8 samples/chip, 4 samples → 1 pooled library
• Flex (small): 1-16 samples → 1 lib, 17-32 samples → 2 libs
• Flex (large): 33-50 samples → 1 lib, 51-96 samples → 2 libs
• Timeline: Shown per batch. Multiple batches processed sequentially (time × batches).

Assay Selection

Method A

Cell surface protein
Intracellular protein
V(D)J (TCR/BCR)
CRISPR guide

Method B

Cell surface protein
Intracellular protein
V(D)J (TCR/BCR)
CRISPR guide

Method C

Cell surface protein
Intracellular protein
V(D)J (TCR/BCR)
CRISPR guide

Comparison

Specification Method A Method B Method C

Workflow Overview (Normalized Phases)

All workflows use the same 5-phase structure. Assay-specific steps appear within each phase.

Workflow Phase Method A Method B Method C

University of Chicago | Human Immunology and Multiplex Imaging (HIM) Facility

Calculator v2.0 | Generated January 16, 2026 | For research use only

Pricing and timelines are estimates. Contact HIM for current rates and consultation.